restriction fragment

Thân thiện

A scientist examines a restriction fragment on a gel electrophoresis plate.

Definition

Noun: A restriction fragment is a specific piece of DNA that is generated when a longer DNA molecule is cut (cleaved) at precise locations by a protein called a restriction enzyme.

Usage

This term is used primarily in the fields of molecular biology, genetics, and biotechnology. It describes the product of a laboratory technique used to analyze and manipulate DNA. - Scientists separated the restriction fragments using gel electrophoresis to analyze the genetic sample. - The size of the restriction fragment helps identify specific genes or genetic variations.

Advanced Usage

• "to map restriction fragments": To determine the order and locations of different restriction fragments along a chromosome or DNA strand, creating a physical map.
  • The team worked to map the restriction fragments of the bacterial genome.

Variants and Related Words

• Restriction Enzyme (n): The protein that cuts the DNA to create restriction fragments.
• Restriction Site (n): The specific DNA sequence recognized and cut by a restriction enzyme.
• Restriction Fragment Length Polymorphism (RFLP) (n): A technique that analyzes variations in the length of restriction fragments to identify genetic differences between individuals.

Synonyms

• DNA fragment: A more general term for any piece of DNA.
• Cleavage product: A general term for any molecule resulting from a cleavage (cutting) reaction.

Related Phrases

• "to run restriction fragments on a gel": A common laboratory phrase meaning to separate restriction fragments by size using gel electrophoresis.
  • After the digestion, the next step is to run the restriction fragments on a gel.

Học thuật

Noun

1. the fragment of DNA that is produced by cleaving DNA with a restriction enzyme